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Description
Description of the bug
Hi,
Unfortunately, there is no specific guidance for creating samplesheets for the methylarray module on nf-core or GitHub. The available samplesheet guidance is for FASTQ files, which are not the correct input for this module. I’ve tried several samplesheets to run the pipeline, but none of them have worked.
Command used and terminal output
input in the shell:
#!/bin/bash
#SBATCH --job-name=methylarray
#SBATCH --output=nextflow_methylarray.log
#SBATCH --ntasks=1
#SBATCH --cpus-per-task=16
#SBATCH --mem=64G
#SBATCH --time=24:00:00
nextflow run nf-core/methylarray \
--input samplesheet2.csv \
--idat_dir /lustre/home/mahdianis/aging_HF/idat_files \
--outdir ./methylarray_output \
-profile singularity \
-r dev
output:
1. ERROR ~ Error executing process > 'NFCORE_METHYLARRAY:METHYLARRAY:PREPROCESS (/lustre/home/mahdianis/aging_HF/SampleSheet2.csv)'
Caused by:
Process NFCORE_METHYLARRAY:METHYLARRAY:PREPROCESS (/lustre/home/mahdianis/aging_HF/SampleSheet2.csv) terminated with an error exit status (1)
2. Command error:
The following object is masked from ‘package:MatrixGenerics’:
rowMedians
The following objects are masked from ‘package:matrixStats’:
anyMissing, rowMedians
3.Error in list.files(path = base, pattern = "_Grn.idat$", recursive = recursive, :
invalid 'path' argument
Calls: read.metharray.exp -> list.files
Execution haltedRelevant files
System information
Nextflow version: 25.04.2.5947
Hardware: HPC (Athena cluster)
Executor: slurm
Container engine: Singularity
OS: CentOS Linux
Version of nf-core/methylarray: dev
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bugSomething isn't workingSomething isn't working